The subcellular organelle not bound by a single membrane is

Golgi apparatus
Endoplasmic Reticulum
Mitochondria
Lysosomes

The correct answer is D. Lysosomes.

Lysosomes are single-membrane-bound organelles that contain hydrolytic enzymes that can break down proteins, carbohydrates, lipids, and nucleic acids. They are involved in the process of autophagy, which is the breakdown of damaged or unwanted cells. Lysosomes are also involved in the immune response, as they can destroy foreign cells and bacteria.

Golgi apparatus is a stack of flattened sacs that are involved in the modification and packaging of proteins and lipids. It is also involved in the formation of lysosomes.

Endoplasmic reticulum is a network of tubules and sacs that is involved in the synthesis of proteins and lipids. It is also involved in the transport of molecules within the cell.

Mitochondria are double-membrane-bound organelles that are the powerhouses of the cell. They are involved in the production of ATP, which is the energy currency of the cell.