The oxygen evolved during photosynthesis comes from splitting of

The oxygen evolved during photosynthesis comes from splitting of

[amp_mcq option1=”water” option2=”carbon dioxide” option3=”oxygen” option4=”light” correct=”option1″]

This question was previously asked in
UPSC NDA-2 – 2018
During photosynthesis, the oxygen gas evolved as a byproduct comes from the splitting of water molecules (photolysis).
– The overall equation for photosynthesis is 6CO₂ + 6H₂O + Light Energy → C₆H₁₂O₆ + 6O₂.
– Experiments using isotopic tracing (specifically, using water labeled with the heavy oxygen isotope ¹⁸O) demonstrated that the ¹⁸O appeared in the evolved oxygen gas, while using carbon dioxide labeled with ¹⁸O resulted in the ¹⁸O appearing in the glucose product, not the oxygen gas.
Water splitting occurs during the light-dependent reactions of photosynthesis, providing electrons for the electron transport chain, protons for the proton gradient used in ATP synthesis, and oxygen as a waste product. The oxygen atoms in glucose are derived from carbon dioxide.