The correct answer is A. One Long Two Short.
The loco pilot will sound one long and two short whistles to inform the guard of a defective stop signal. This is the standard procedure for informing the guard of a problem, and it allows the guard to take appropriate action to ensure the safety of the train.
Option B is incorrect because it is the wrong number of whistles. Option C is incorrect because it is the wrong order of whistles. Option D is incorrect because it is not a valid option.