The correct answer is: C. First 64k above conventional.
The HIGH Memory Area (HMA) is the first 64 kilobytes (KB) of extended memory above the 1 megabyte (MB) boundary. It can be accessed by DOS programs using the XMS API.
The last 64KB of conventional memory is reserved for the BIOS and cannot be used by DOS programs. The last 64KB of extended memory is not accessible to DOS programs without using a third-party memory manager.
The first 64KB of extended memory is the HMA, which can be accessed by DOS programs using the XMS API.