The correct answer is: b) Interference in traditional customs.
U Tirot Sing was a Khasi chief who led an uprising against the British in the early 1830s. The uprising was caused by the British interference in traditional Khasi customs, such as the inheritance of property by women. The British also tried to impose their own laws and customs on the Khasis, which led to further resentment.
The other options are incorrect. Option a) is incorrect because the British did not impose high taxes on the Khasis. Option c) is incorrect because the British did not construct new roads through tribal territories. Option d) is incorrect because the British did not persecute the Khasis for their religion.
The uprising was unsuccessful, and U Tirot Sing was killed in battle. However, the uprising is still remembered by the Khasis as a symbol of their resistance to British rule.